Imagej quantification western blot4/11/2024 H2AX becomes phosphorylated on its residue serine 139 in cells when DNA DSBs occur 18. H2AX is one of the most conserved variants of the histone H2A and accounts for 2–25% of the H2A protein 21. In addition, manual or semi-automated assessment of DSBs can be used to elucidate the protective effect of compounds against genomic damage 15, assess the DNA repair process of cells 1, or even establish the endogenous load of DNA DSBs within the cells 16.ĭNA DSBs trigger a DNA damage response that is characterized by activation of DNA repair mechanisms and phosphorylation of the histone protein component H2AX to form phosphorylated H2AX (γ-H2AX) 17, 18, 19, 20. Effective and efficient quantification of DSBs is critical for managing the effective dose for radiation therapy, identifying exposure to cytotoxic agents or environmental factors, and assessing the pharmacodynamics of chemotherapeutics. However, given the detrimental effects of DNA DSBs on cell viability and the induction of mutated cells, it is critical to quantify them effectively to better inform treatment decisions. The ability of different forms of irradiation to induce DNA DSBs has been harnessed in radiotherapy treatments to kill cancer cells 13, 14. Recent studies using a very fast CRISPR Cas 9 system to induce DNA DSBs have tracked their formation over time and length scales 12. Exogenous factors inducing DNA DSBs include cytotoxic chemical agents 2, 3, 4 and environmental 5 and physical factors such as radiation 6, 7, 8, 9 and heat 10, 11. They are difficult to repair and can lead to genotoxicity, cell death, and misrepaired DNA DSBs with potential for neoplastic progression. DNA DSBs present the most serious threat to the cell. Similar content being viewed by othersĭNA damage in cells can be triggered by endogenous mechanisms, exogenous factors, or a combination of both, resulting in a multitude of alterations including DNA base modifications, single-strand breaks, and double-strand breaks (DSBs) 1. These approaches have the potential to improve screening of compounds that either enhance DNA damage or protect against its deleterious effects. Lanthanide based immunodetection of γ-H2AX offers superior detection and a user-friendly workflow. Comparative bulk fluorescent signals achieved only micromolar sensitivity. Time resolved fluorescence detection of europium as a chelated complex enabled quantitative measurement of γ-H2AX foci with nanomolar resolution. Etoposide induced DNA damage in A549 carcinoma cells was compared across all test platforms. For comparison, standard fluorescence detection was employed and analyzed either by bulk fluorescent measurements or by direct foci counting using BioTek Spot Count algorithm and Gen 5 software. The novelty of this work is the application of a time-resolved fluorescence assay using dissociation-enhanced lanthanide fluorescence immunoassay for quantitative measurements of γ-H2AX. Traditional γ-H2AX detection involves counting individual foci within individual nuclei. Phosphorylation of the histone protein H2AX to form γ-H2AX foci directly represents DNA double-strand break formation.
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Arduino mega 2560 special pins4/11/2024 There are 16-analog pins included on the board which is marked as A0-A15. The term AREF stands for Analog Reference Voltage which is a reference voltage for analog inputs This will give you to modify the programming skills in real time. This LED can be operated based on the high and low values of the pin. This Arduino board includes a LED and that is allied to pin-13 which is named as digital pin 13. Providing LOW value, rising or falling edge or changing the value to the interrupt pins. These pins produce interrupts by a number of ways i.e. The external interrupts can be formed by using 6-pins like interrupt 0(0), interrupt 1(3), interrupt 2(21), interrupt 3(20), interrupt 4(19), interrupt 5(18). For serial 0, it includes Tx (1) and Rx (0), for serial 1, it includes Tx(18) & Rx(19), for serial 2 it includes Tx(16) & Rx(17), and finally for serial 3, it includes Tx(14) & Rx(15). The serial pins of this board have four combinations. Tx indicates the transmission of information whereas the RX indicates receive data. The serial pins of this board like TXD and RXD are used to transmit & receive the serial data. However, the output voltage through this pin to the board will be automatically set up to 5V. The voltage provided by the power jack can be accessed through this pin. The range of supplied input voltage to the board ranges from 7volts to 20volts. When we compare with other types of Arduino boards, these boards are physically superior. These boards offer flexible work memory space is the more & processing power that permits to work with different types of sensors without delay. By using this board, the Arduino mega projected can be designed. All analog pins of this board can be used as digital I/O pins. Every pin of this board comes by a particular function which is allied with it. The pin configuration of this Arduino mega 2560 board is shown below. The USB host chip used in this is MAX3421E.The electrically erasable programmable read-only memory (EEPROM) is 4 KB.The static random access memory (SRAM) is 8 KB.Flash Memory like 256 KB where 8 KB of flash memory is used with the help of bootloader.DC Current for each input/output pin is 40 mA.The digital input/output pins are 54 where 15 of these pins will supply PWM o/p.The input voltage will range from 6volts to 20volts.The recommended Input Voltage will range from 7volts to 12volts.The operating voltage of this microcontroller is 5volts.The specifications of Arduino Mega include the following. These boards work with every existing shield although can adjust to latest shields which utilize these extra pins. Another pin is not associated & it is kept for upcoming purposes. One pin is the IOREF that permit the shields to adjust the voltage offered from the Arduino board. Additionally, there are two latest pins located near the RST pin. The SCL & SDA pins of Mega 2560 R3 board connects to beside the AREF pin. This board can be protected from the unexpected electrical discharge by placing a base plate. So, the power supply of this board can be done by connecting it to a PC using a USB cable, or battery or an AC-DC adapter. This board mainly includes everything which is essential for supporting the microcontroller. It includes digital input/output pins-54, where 16 pins are analog inputs, 14 are used like PWM outputs hardware serial ports ( UARTs) – 4, a crystal oscillator-16 MHz, an ICSP header, a power jack, a USB connection, as well as an RST button. The microcontroller board like “Arduino Mega” depends on the ATmega2560 microcontroller. Gangster tattoo fonts and lettering4/11/2024 The allure of Westcoast lies in its ability to seamlessly blend grit and elegance, capturing attention and adding an element of rebellion to any design. This font exudes a distinctive gangster style, making it an ideal choice for tattoo designs, tattoo studio logos, shirt designs, posters, and branding projects. Image Credit: Envato ElementsĮnter the world of edgy sophistication with the Westcoast Gangster Font. Westcoast Gangster Font Westcoast Gangster Font. So, if you’re looking for an edgy and stylish tattoo font, consider choosing a gangster one for your next design! You won’t be disappointed with the results.Ģ5. And because these fonts are so popular, you’ll have no trouble finding plenty of great examples and inspiration online. Whether you’re looking for something traditional or modern, there’s sure to be a font that fits your needs. Additionally, gangster tattoo fonts often have a lot of character and personality, which can help reflect your unique style.Īnother great reason to choose a gangster tattoo font is that they tend to be very versatile. For one, these fonts tend to be bold and eye-catching, which can help you make a statement with your tattoo. You might choose a gangster tattoo font for your next ink design for many reasons. No matter what you’re looking for, there’s sure to be a gangster tattoo font that’s perfect for you! Why Choose The Best Gangster Tattoo Fonts? However, there are also some more modern takes on this style that can add a touch of sophistication to your tattoo. These fonts are often inspired by the traditional tattoos of gangsters and prisoners, which means they tend to be bold and eye-catching. Gangster tattoo fonts are popular for those looking to add an edgy and tough look to their ink designs. So whether you’re looking for something traditional or modern, we have you covered! What Are Best Gangster Tattoo Fonts? Luckily, there are plenty of great options for you to choose from! This blog post will take a look at some of the best gangster tattoo fonts available today. You want something tough and edgy yet stylish and timeless. When choosing the best gangster tattoo fonts, you want something that will capture the essence of what you’re trying to represent. Printable position chart for trombone4/11/2024 Check Details Trombone slide position chart dansr Trombone brassTrombone with f attachment basic slide chart printable pdf download Trombone slide chart position music sheet fingering band positions kids instrument escala charts música instruction wordpress trombón cromatica partituras teachersTrombone slide positions chart : r/brass. Trombone slide position chart.jpg (606×716)Trombone fingering/slide position chart and flashcards Trombone positions chart and how the trombone worksSlide position chart for trombone. 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Trombone chart positions Slide trombone chart Bass trombone slide position chart Trombone intonation triads These are supplemented by MP3 recordings allowing the student to hear what the compositions sound like and to use as play-along accompaniment pieces.Trombone slide position chart by extendabone llc Trombone with f attachment basic slide chart download printable pdf Download trombone slide position chart google play softwares trombone positions 2 0 These collections are for Low Brass Ensembles or for any of the three low brass instruments (trombone, euphonium or tuba). Low Brass Ensemble scores and sheet music compositions of popular melodies
Win 10 iso loader4/11/2024 For tips on locating your product key, check Find your Office 2010 product key or Enter the product key for your Office 2007 program. If you have Office 2010 or earlier and choose to perform a clean install of Windows 10, you will need to locate your Office product key. For more information, check How to upgrade to Windows 10 on new devices that include Office 365. If the problem isn't fixed, run the following commands: bcdedit /export c:\bcdbackup attrib c:\boot\bcd -r -s -h ren c:\boot\bcd bcd.old bootrec /rebuildbcd. Restart the computer to check whether the problem is fixed. To redeem your copy of Office, please see Download and install Office 365 Home, Personal or University on your PC. To do this step, run the following command: Bootrec /ScanOS. If you just purchased a new device that includes Office 365, we recommend redeeming (installing) Office before upgrading to Windows 10. Please see the Volume Licensing Service Centre for more information. Windows 10 Enterprise isn’t available here. To check which edition you're currently running, go to PC info in PC settings or System in Control Panel and look for Windows edition. You should also choose the same edition of Windows. Windows 10 22H2 Full merupakan versi windows 10 dengan rillis beberapa versi windows, yakni versi Home, Education, Pro dan Pro Workstation dari based Windows 10 Consumer editions update July 2023. To see what language you're currently using, go to Time and language in PC settings or Region in Control Panel. You'll need to choose the same language when you install Windows 10. We also recommend that you visit your PC manufacturer's website for info about updated drivers and hardware compatibility. See the system requirements before installing Windows 10. To check this on your PC, go to PC info in PC settings or System in Control Panel, and look for System type. You’ll create either the 64-bit or 32-bit version of Windows 10 that’s appropriate for your CPU. When burning a DVD from an ISO file, if you are told the disc image file is too large you will need to use Dual Layer (DL) DVD Media.Ĭheck these things on the PC on which you want to install Windows 10:.DAEMON Tools Lite is the leading software that allows you to mount, create, and emulate disk images with. We recommend using a blank USB or blank DVD, because any content on it will be deleted. Convert and mount ISO images with DAEMON Tools Lite. A blank USB flash drive with at least 5 GB of space or blank DVD (and DVD burner) if you want to create media. Sufficient available data storage for the download, on a computer, USB or external drive.An internet connection (internet service provider fees may apply). Free template for gantt chart in excel4/11/2024 Take a deeper dive into everything you need to know about Gantt charts, including how to use them, their limitations, and the qualities that make them special. It’s also a great way to ensure that you won’t run into overlap! Ideally, your tasks should be staggered in a sort of waterfall-like fashion on your project timeline, but if you notice a nearly vertical stack of bars during the same stretch of time, it’s time to bump some due dates. This helps keep every team member on the same page and helps you set realistic goals for future project planning. With each task stretched and stacked along your timeline, you can easily determine your team’s progress, the amount of work remaining, and the project’s current status. The length of the bar then shows how long you have to complete the task. Each task is displayed as a horizontal bar and where it begins represents the start date of that specific task. 3 Benefits of Using Gantt chart TemplatesĪ Gantt chart template graphically represents all the tasks and deliverables in an ongoing project.How to Use a Gantt Chart Template in ClickUp.IT Roadmap Gantt Chart Template by ClickUp Uses Acceptance Testing Gantt Chart Template by ClickUp Business Planning Gantt Chart Template by ClickUp Simple Gantt Chart Template by Google Sheets Waterfall Management Gantt Chart Template by ClickUp Product Launch Checklist Template by ClickUp Construction Management Gantt Chart Template by ClickUp Building Web Pages Gantt Chart Template by ClickUp Blog Management Gantt Chart Template by ClickUp Not only will we break down every key element of Gantt chart project management and its benefits, but we’ve also added ten of our favorite templates with tips on how to get the ball rolling (at top speed). If you’re already knee-deep in another project management process but are Gantt chart-curious about how this other methodology could benefit your team, you’re in the right place, my friend. Gantt charts are a popular and highly visual methodology used to display your tasks against the time allotted to complete them.Īt a glance, you can easily communicate any overlap between multiple tasks, all of the tasks involved in the project, key deadlines, and the start and end date of the entire project. Gantt charts are a great place to start if you find yourself in this boat and we have the best templates to get you started! While it almost guarantees that a perfect-fit process exists for every team, it also adds a bit of challenge with the trial and error search needed to secure your preferred approach. The ever-growing selection of project management software and methodologies is what makes life so exciting (and a little stressful) for managers. You might still find what you’re looking for, but not without spending a fair amount of time sifting through the mess first. Managing projects without Gantt charts is like trying to find something in an overstuffed suitcase. Star wars scenery painter4/11/2024 The series is targeted at young audiences and their families, and is set during the High Republic era, following a group of younglings as they learn the ways of the Force, including compassion, self-discipline, team work and patience, to become Jedi Knights. In May 2022, a Young Jedi Adventures animated series was announced at Star Wars Celebration. Main article: Star Wars: Young Jedi Adventures The show was later revived for an additional final season that premiered on February 21, 2020, on the streaming service Disney+. The film and series were included in the canon established in 2014. The chronological storyline order was released after the series had initially finished airing. The remaining unaired episodes were released on Netflix as "The Lost Missions". The series marked the beginning of Dave Filoni's involvement in Star Wars animation projects.Īfter Disney's acquisition of the Star Wars franchise, The Clone Wars was cancelled in 2014 before its intended final episodes were completed. It focuses mainly on the Jedi characters of Anakin Skywalker and Obi-Wan Kenobi, as well as Anakin's Padawan apprentice, Ahsoka Tano (an original character created by Filoni for the series), with other characters from the live-action films in supporting roles. The series is set between Attack of the Clones and Revenge of the Sith of the prequel trilogy. The Clone Wars (2008–2020) was introduced through a 2008 animated film of the same name. George Lucas created his own animation company, Lucasfilm Animation, and used it to produce his first in-house Star Wars CGI-animated series, using Cartoon Network's 2003 Clone Wars micro-series as a "pilot". Main article: Star Wars: The Clone Wars (2008 TV series) Dave Filoni served as supervising director for both The Clone Wars and seasons 1, 2, and 4 of Rebels, before being promoted to oversee the development of all future Lucasfilm Animation projects in 2016. Additional live-action series include Obi-Wan Kenobi, Andor, and The Acolyte. The first live-action series in the franchise, The Mandalorian, premiered on Disney+ in November 2019 and spawned two spin-offs set in the same timeline, The Book of Boba Fett and Ahsoka, as well as another set around the same era, Skeleton Crew. The first young audience animated series, Young Jedi Adventures, set during the High Republic era, premiered in May 2023. Two animated anthology series have been produced: Visions in 2021 and Tales of the Jedi in 2022. An additional spin-off series from The Clone Wars, titled The Bad Batch, debuted on Disney+ in 2021. Two additional half-hour animated series were created: Rebels, which ties into the original trilogy, and Resistance, which ties into the sequel trilogy. After Disney's acquisition of Lucasfilm, only The Clone Wars was kept in the canon of continuity of the episodic Star Wars films. Further animated series began to be released in the 2000s, the first two of which focused on the prequel trilogy-era Clone Wars. Two animated series were released in the mid-1980s. The Star Wars franchise has spawned multiple films and television series. Learn about their backgrounds, achievements, and personalities on Pompedia, the online encyclopedia of cheerleading. Only the beginning, this 13 th annual edition includes more than 100 photos of America’s Sweethearts photographed in and around beautiful. Featured on the cover is Kat, a fourth-year DCC veteran, basking in the glow of a breathtaking sunset. The Cowboys didn’t allow cheerleaders to drink or be photographed with someone drinking. Meet the 2016-17 Dallas Cowboys Cheerleaders, the world-famous squad that performs at every home game and represents the team in various events. Often imitated, never equaled, the 2022 Dallas Cowboys Cheerleaders Swimsuit Issue is now available. Several cheerleaders were angered when Jones told them they had to appear in beer commercials. According to the Tulsa World, Jones wanted the cheerleaders to trade their current uniforms - long-sleeved shirts, a vest, and shorts - for biker shorts and a halter top.Ĭheerleaders also had to work parties as hostesses and fraternize with players. Several of those ideas caught Cowboys cheerleaders by surprise. When Jerry Jones bought the Dallas Cowboys in 1989, he did what many new owners and bosses do: present ideas about the future of the company. Jerry Jones wanted ‘America’s Sweethearts’ to build relationships with players Dallas Cowboys cheerleaders didn’t take kindly to Jerry Jones’ ownership in 1989. Nicknamed ‘America’s Sweethearts,’ the squad was as iconic as Tom Landry and Roger Staubach in Dallas. After a scantily-woman named Bubbles Cash drew attention during a 1967 game, Cowboys general manager Tex Schramm wanted all of his cheerleaders to wear similar outfits.īy 1969, the Cowboys cheerleading team only featured younger women in revealing outfits. What the Cowboys did, though, was have cheerleaders wear less clothing than they had in the past. That title belonged to the Colts, who first utilized cheerleaders in 1954. The Cowboys weren’t the first team to use cheerleaders when the franchise debuted in 1960. The cheerleaders that fans see and, well, cheer for during the NFL season partly originated from the Dallas Cowboys’ squad. Meal planning printable sheet4/11/2024 If you need more than only one template, please consider the print-ready full-sized meal planners. Download meal planning template now to start planning meals easy. 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Download printable weekly planners and weekly planner PDF (for Excel templates use google) to start planning today with beautiful weekly calendars 2024 2025. They are customizable, functional, simple and don’t require too much hassle to start working with. If you are someone who likes to plan with pen and paper and values unlimited flexibility, make sure to consider free weekly calendars and these weekly planner printables. It’s time to employ the daily productivity tools designed to help you manage your time better, become really productive, plan your weekend schedule, keep track of holidays and stay organized throughout the year. Therefore, it’s crucial to master everyday planning and do it the right way. Today, it’s impossible to conquer the hectic world without being organized every day. Here are the best weekly schedule templates you can customize and download. This collection is full of ready-made professionally-designed blank weekly calendars. While downloading printable weekly calendars, you can also choose the format and the start day you prefer: Monday or Sunday. These templates will help you keep track of your chores, tasks and appointments, organize easily your personal and class schedule or work schedule. If you prefer weekly planning, the weekly layout is the right choice for you. A much more flexible tool is a printable planner template that works for all: students, teachers, busy moms and professionals. The good news is that there is a better way to keep planning with pen and paper in 2024. They often lack flexibility and room for personalization. Most of the planners come with a structure that might work perfectly well for one person and be totally useless for another one. If you are a stationery aficionado and like planning your day the old way with pen and paper, you know how difficult it might be to find the medium that can help you stay organized and productive. Welcome to the collection of the best 2024 - 2025 weekly planners templates with different planning pages and layouts. Fall wedge boots 20174/11/2024 My main reason for taking the course is a discount from my insurance company. It made more sense to me to practice during the day light. I had the option of taking it 7 am to 3 pm or 3 pm to 11 pm. MSF wanted to keep a nationwide curriculum. Fall’s Best New Boots Have Arrived 52 of the Season’s Most Covetable Pairs. Apparently the CA CHP wanted more control over what was being taught and MSF didn't even bid the last time the CHP put it out for bid. At $28 plus tax I thought thy were a good deal. I can't believe how much better gloves are that are made specifically for motorcycles. The motorcycle gloves I bought the same day from a dealer are awesome. Do I really need to use them every time? Perhaps someone with more experience can explain why or if I really need them. So I bought an inexpensive pair since I have to have them for the course, but I am thinking that I don't really need to use them every time I ride. Apparently the thought process is that they are needed to keep your ankles protected from the hot muffler or moving motorcycle parts. The Fall Boot Guide 2017 Sharing all of my top picks, some helpful tips and tricks and what you need to know about boot trends for this season. The instructions from the course say that I have to bring, among other things, sturdy over the ankle boots. Your favorite brands, such as Ganni and Jeffrey. Western-inspired boots are back but with more color. Now that spring has finally arrived, we're ready to depart from our old ways and preconceived notions and embrace the trend for all it's worth.Īhead, see where we've charted 2023's wedge shoe trend from the runways to fashion people's feeds and then shop the pairs that are defining the movement.I am taking the California Motorcycle Safety course this Thursday and Friday. Buckle up as we ride off into fall with the coastal cowgirl trend. With sculptural shapes and streamlined non-platform silhouettes, we'd even go so far as to say that wedges are *the* shoe trend of the moment, and the retailers carrying these styles you're about to see below are proving me right. Even the fall 2023 collections echoed this sentiment, with Ferragamo, Givenchy, and Missoni showing sleek wedges via slingback and Mary-Jane styles that convinced us this isn't just a one-off shoe moment-it's a whole trend movement. Thanks to Bottega Veneta and Khaite, 2023's wedges are defined by cutout heel shapes that could be works of art in their own right, and many other designers are following suit. Find the perfect boots, slippers, sneakers, and sandals to complete your look - from statement fluffy platforms to cozy house shoes, we have you covered. The shapes this year are much sleeker, and the designers behind them are much buzzier. Home Women Girls Boys Men Find Us Online. But before you fully freak out, the wedges that are coming back now are far from the chunky platform wedges you used to know. Shop a wide variety of shoes for women: Athletics, Sandals, Casuals, Dress, Boots and Safe-T-Step at Payless. Whether you never stopped wearing them (kudos to you if so) or felt the need to ditch the style immediately post-graduation, everyone has a tumultuous relationship with the shoe style, so there's no denying that a 2023 return is literally shocking news. Not to freak you out, but wedges are officially coming back from the dead. AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |